Advantages:

The purpose of this research laboratory was to learn how to use a spectrophotometer to gauge the amount of sunshine absorbed by simply different concentrations of salicylic acid, assess those concentrations to our not known sample and use the info collected to compile a graph displaying the levels of absorbance with the different concentrations.

Methods and materials:

In this research laboratory we utilized a spectrophotometer, a test out tube filled up with water being used as a blank, half a dozen test pipes with different concentrations of salicylic acid which range from 0 mg/dL to 5 mg/dL and one test conduit with a mysterious concentration of salicylic chemical p. We set the spectrophotometer to a wavelength of 540 nm. All of us adjusted the transmittance to 0%. Next we put the test pipe with the water into the spectrophotometer and altered the transmittance dial to 100%. We removed test tube made up of the water and replaced that with the first concentration of 0 mg/dL. We noted the absorbance and repeated the test to get a total of 5 psychic readings. We did this for each and every concentration. We then determined the average of each and every concentration's absorbance readings and plotted the averages on to the graph.

After we had gathered the data pertaining to our known concentrations we all then repeated the procedure pertaining to our unidentified concentration. We all again took the average and plotted that on the absorbance curve to look for the concentration of the unknown.

Observations and Info:

Adjusted Standard| Absorbance Reading 1| Absorbance Reading 2| Absorbance Reading 3| Absorbance Studying 4| Absorbance Reading 5| AverageAbsorbance Reading| 0 mg/dL| 0. 007| 0. 010| 0. 007| 0. 005| 0. 006| 0. 007

zero. 5 mg/dL| 0. 032| 0. 036| 0. 037| 0. 041| 0. 038| 0. 037| 1 . 5 mg/dL| zero. 098| zero. 100| zero. 098| 0. 099| 0. 096| 0. 098| installment payments on your 5 mg/dL| 0. 150| 0. 149| 0. 153| 0. 154| 0. 150| 0. 151| 3. a few mg/dL| zero. 234| zero. 239| 0. 237| 0. 250| zero. 229| zero. 238| five. 0 mg/dL| 0. 286| 0. 287| 0. 288| 0. 292| 0. 291| 0. 289| Unknown A| 0. 241| 0. 238|...